Restore Total Gut Health: Microbiome Labs Testing Kit with Megasporebiotic, Megapre and Megamucosa

Achieving Total Gut Restoration: The Power of Recondition, Reinforce, and Rebuild (3Rs)

Our colon is not appreciated or understood by many people in our population. Do you realize that length of the colon in most adults is only 6 to 9 feet, yet because of the convoluted projections called villi the surface area when fully expanded would cover the area of an entire tennis court! Inside all these microscopic canyons and crevices is where the real health or sickness for our body and brain takes place. Specifically what the colon is doing in this area is managing what minerals to keep and what toxins to throw away. Fortunately, we have microscopic helpers to assist us along the way. When these helpers are overwhelmed by too many toxins and too many microscopic enemies, then the colon becomes inflamed and dysfunctional.


Unfortunately, many people have been plagued with digestive problems for years. Leaky gut that cause allergic food reactions, some have diarrhea, others experience gas, ulcers, acid reflux the list goes on and on and it seems so many people suffer from digestive issues these days.

So, lets talk about the cause. We have to ask the question Why are people having so many different digestive dysfunctions these days? What do they need?


More than anything they need a balance of the microbiome. But then many people still don't know about the microbiome. What is the microbiome?


To answer this important question Dr. Johnny relied on the National Institute of Health, particularly their division of Environmental Sciences:


The microbiome is the collection of all microbes, such as bacteria, fungi, viruses, and their genes, that naturally live on our bodies and inside us. Although microbes are so small that they require a powerful microscope to see them, they contribute in big ways to human health and wellness. They protect us against pathogens, help our immune system develop, and enable us to digest food to produce energy.


TOTAL GUT RESTORATION

Because the microbiome is a key interface between the body and the environment, these microbes can affect health in many ways and can even affect how we respond to certain environmental substances. Some microbes alter environmental substances in ways that make them more toxic, while others act as a buffer and make environmental substances less harmful.


What Are Short-Chain Fatty Acids and How Does This Protocol Encourage Their Production?


Let’s take a closer look at short-chain fatty acids (SCFAs), since they’re one of the colon’s MVPs when it comes to maintaining a happy, healthy gut. You can think of SCFAs like acetate, propionate, and butyrate as tiny chemical messengers created when friendly gut microbes break down certain fibers and prebiotics from your diet. These helpful compounds play a starring role in nourishing the cells lining your intestinal wall, supporting a strong barrier, and even calming your immune system’s overzealous responses.


But here’s the catch: when our gut is out of balance, the population of good bacteria takes a hit, and SCFA production drops off. That’s where a strategy like the Total Gut Restoration protocol shines. By introducing the right combination of probiotics and specialized prebiotics, this approach helps encourage a more diverse landscape of microbes especially in those twisty, turny areas of the colon where digestion really happens.


With microbial diversity back on the rise, your “good guy” bacteria are better equipped to churn out those essential SCFAs, restoring a harmonious environment for both your gut and your overall well-being.


Today many people complain some different gut issues whether it is ulcers, colitis, diarrhea, gluten intolerance and so many more uncomfortable and disruptive issues. But interestingly Dr. Johnny found a unique and viable solution for all gut issues with the 3Rs from Microbiome Laboratories. The 3Rs stand for Recondition, Reinforce, Rebuild and these are 3 specific products to get the microbiome back to balance and good health.

TOTAL GUT RESTORATION (3Rs)

Clinical Evidence Supporting Spore-Based Probiotics, Prebiotics, and Mucosal Support

If you’re still wondering whether these “microscopic helpers” genuinely pull their weight, let’s take a quick jaunt through the scientific evidence behind spore-based probiotics, prebiotics, and supplements designed to reinforce the gut lining. When Dr. Johnny dove into the research, he found these products far from mere health fads they are backed by a growing body of rigorous clinical studies.


What the Studies Show


Researchers have explored spore-based probiotics, prebiotics, and mucosal support from multiple angles, including outcomes like:


  • Digestive Comfort and Regularity
    Multiple human and animal studies show that spore-based probiotics can ease occasional abdominal discomfort, encourage regular elimination, and generally support smoother digestive function.
  • Immune System Support
    These beneficial microbes bolster the gut’s immune activity, assisting the body in defending against unwanted invaders and helping the immune system maintain its delicate balance.
  • Reinforcement of the Intestinal Barrier
    Mucosal support compounds (think “the fortifying shield for your gut lining”) have demonstrated the ability to strengthen this natural barrier, making it more resilient against irritants and toxins.
  • Promotion of Microbial Diversity
    A healthy, diverse population of gut bacteria is essential, and studies show prebiotics particularly in combination with spore-based probiotics foster beneficial diversity, which is crucial for long-term digestive and metabolic health.
  • Metabolic & Heart Health
    Some trials reveal that regular use of spore-based probiotics can have favorable effects on things like triglyceride and cholesterol levels bonus points for your heart and energy metabolism.
  • Relief During Antibiotic Use
    When taken alongside antibiotics, spore-based probiotics have helped maintain a healthy balance of gut flora, warding off some of the common discomforts that antibiotic therapy can bring.


Real-World Implications


Brands like Seed, Just Thrive, and Hyperbiotics (to name a few) have been featured in peer-reviewed journals for these exact attributes. Key findings include increased production of short-chain fatty acids (like butyrate and propionate, vital for both gut and brain health), stronger mucosal lining defenses, and a measurable uptick in beneficial microbe populations.


In summary, the science says these microbiome-boosting supplements aren’t just another trip down the supplement aisle they’re validated tools for anyone serious about restoring gut health and whole body well being.

Why Use the Total Gut Restoration 3-Month Protocol?

The Microbiome Labs’ Total Gut Restoration Program Supports Gut Health Recovery. The Total Gut Restoration Kit is Microbiome Lab's cutting-edge gut health recovery regimen and the cost is $365.


Addressing gut health is one of the foremost and vital aspects of optimal well-being, however, gut restoration can be challenging. It can feel like an unconquerable struggle, especially if you have been experiencing digestive or other health issues for a long time.


Fortunately, microbiome testing from Microbiome Labs is at the forefront of converting new research about gut health into high-purity, high-efficacy supplements that get realworld results.


Scientific Foundations: Studies Supporting the Total Gut Restoration Protocol


If you’re reading this and wondering, “But what does the science actually say?” rest assured, there’s plenty of evidence from the academic world to bolster these microbiome-balancing strategies. Over recent years, clinical and laboratory research has delved into the effects of targeted probiotic, prebiotic, and mucosal support supplements (like those found in this protocol) on gut health and overall well-being.


Here’s a snapshot of what scientific studies have uncovered:


  • Strengthens the Intestinal Barrier
    Laboratory models using fecal samples have shown that supplement protocols aimed at reconditioning and rebuilding the microbiome can promote the production of vital short-chain fatty acids (such as butyrate), which are known to support intestinal wall integrity and a healthy immune response.1
  • Supports Comfortable Digestion and Immune Function
    Multiple studies—ranging from 10 days to several months have documented improvements in immune markers, reduced occasional abdominal discomfort, and better elimination patterns. Animal and human studies alike report these shifts correlate with robust probiotic supplementation.2,3
  • Promotes Microbial Diversity and Resilience
    The combination of certain probiotic and prebiotic formulas (“synbiotics”) has been demonstrated to boost both microbial richness and diversity throughout the colon. Enhanced diversity is consistently associated with better digestive health, nutrient absorption, and resilience against invading organisms.4
  • Helps Maintain Healthy Triglyceride and Iron Levels
    Trials lasting several weeks have observed positive shifts in metabolic markers like triglycerides and iron absorption, underscoring the interplay between the gut and whole-body wellness.5,6
  • Supports Cardiovascular and Liver Health
    Some studies even report promising effects on cardiovascular and liver function markers following targeted probiotic protocols, hinting at the far-reaching benefits of a balanced gut microbiome.7,8


What does this all mean? Targeted gut restoration approaches, combining precise probiotic and prebiotic supplementation with support for the gut lining, are not just a hopeful idea they’re grounded in emerging scientific research. This evidence supports why so many practitioners are turning to this comprehensive protocol for those seeking to rebuild their gut from the inside out.


  1. DOI: 10.2217/fmb-2022-0066
  2. DOI: 10.1002/jpen.1851
  3. DOI: 10.3390/nu11091968
  4. DOI: 10.1016/j.ijpx.2019.100021
  5. PMID: 33041703
  6. DOI: 10.3390/nu12123607
  7. DOI: 10.4291/wjgp.v8.i3.117
  8. DOI: 10.3390/nu12030632


Microbiome Labs’ Total Gut Restoration kit contains this power-packed triad of digestive health supplements:


  • MegaSporeBiotic™: A blend of five keystone strains of spore-based probiotic Bacillus bacteria. These rugged spores survive digestion, colonize your gut, and may improve digestion, microbial richness and diversity, resistance to pathogenic organisms, and more.


Unlike many conventional probiotics, this all-spore formula is designed to actively recondition your gut environment helping to maintain a healthy gut barrier and support the growth of beneficial, commensal bacteria. Its robust nature means no refrigeration is required, and the long shelf-life ensures potency remains intact. If you're seeking a broad-spectrum approach to fostering a balanced and resilient gut microbiome, spore-based probiotics like this are a powerful option.


The Benefits of Shelf-Stable, Non-Refrigerated Supplements


One of the unsung heroes in gut health supplementation is a formula that doesn’t chain you to your fridge. If you’re like most people, your supplement routine should fit your life not the other way around.


Here’s why a long shelf life and no refrigeration matter:


  • Convenience: Whether you’re traveling across the globe, headed out for a long weekend, or just running late for work (again), you don’t have to worry about lugging around a cooler or dealing with spoiled capsules. Supplements that are shelf-stable give you ultimate freedom just toss them in your bag or desk drawer and you’re good to go.
  • Consistency: Let’s face it, most of us have forgotten a supplement in the back of a cabinet at some point. With a long shelf life, you aren’t stuck racing against an expiry date. You can keep your gut health routine steady for months, knowing your investment isn’t going to waste.
  • Reliability: Some probiotics on the market lose potency fast if they’re not chilled, especially during shipping or storage. But robust, shelf-stable strains maintain their strength regardless of the season or your kitchen’s microclimate. That means you can trust each serving to deliver what your gut needs, every time.
  • No More Guesswork: No need to double-check if your supplement’s a science experiment in the fridge or fret about an interrupted cold chain during delivery. With spore-based and other shelf-stable options (think of brands like Hyperbiotics or Just Thrive), peace of mind comes standard.


In summary: choosing a supplement that keeps its cool under pressure without refrigeration means more flexibility, less waste, and reliable support for your gut, wherever life takes you.

MegaSporeBiotic: Serving Size and Ingredients


For those considering MegaSporeBiotic as part of the Total Gut Restoration protocol, here’s what you need to know about how to take it and what’s inside each capsule.


How to Take MegaSporeBiotic:

  • Week 1: Start slowly with 1 capsule every other day.
  • Week 2: Increase to 1 capsule daily.
  • Week 3 and beyond: Take 2 capsules each day, always with a meal. This gradual introduction helps your gut adjust comfortably to the beneficial probiotic strains.


Building Your Gut Support Routine


After your gut has had a chance to acclimate to the probiotic, you can begin layering in complementary supplements for even more comprehensive support:


Add a Prebiotic


  • Week 5: Introduce a prebiotic supplement by taking 3 capsules daily.
  • Week 6 and beyond: Increase to 6 capsules daily, with or without food, alongside your probiotic routine.


Add a Mucosal Support Supplement


  • Week 9: Once you’re comfortable with both the probiotic and prebiotic, start a mucosal support supplement 3 capsules daily.
  • Week 10 and beyond: Increase to 6 capsules daily, with or without meals.


This gradual, stepwise approach gives your digestive system time to adapt at each stage, optimizing results while minimizing any discomfort. Always consult your healthcare provider for personalized guidance.


Serving Information:

  • Full serving size is 2 capsules daily, which delivers a potent dose of spore-based probiotics.


What’s Inside:
MegaSporeBiotic features a proprietary blend of five Bacillus species. These robust, spore-forming probiotics are designed to survive the harsh journey through your stomach and successfully colonize your gut:

  • Bacillus indicus (HU36™)
  • Bacillus subtilis (HU58™)
  • Bacillus coagulans (SC-208)
  • Bacillus licheniformis (SL-307)
  • Bacillus clausii (SC-109)


The formula is thoughtfully designed for sensitive individuals, containing no eggs, dairy, gluten, nuts, soy, or wheat. The only additional ingredients are plant-based: pure cellulose and a vegetable capsule, ensuring compatibility with various dietary restrictions.


Microbiome Labs’ Total Gut Restoration kit contains this power-packed triad of digestive health supplements:


  • MegaSporeBiotic™: A blend of five keystone strains of spore-based probiotic Bacillus bacteria. These rugged spores survive digestion, colonize your gut, and may improve digestion, microbial richness and diversity, resistance to pathogenic organisms, and more.

  • MegaPre™: This trifecta of precision prebiotic "oligosaccharides" targets your beneficial gut microbes, giving them the fuel they need to produce essential metabolites and postbiotic substances that are good for your health.

  • MegaMucosa™: Contains immunoglobulins (mostly IgG) which bind to toxins like bacterial lipopolysaccharides (LPS), along with several key amino acids like L-serine, L-proline, L-threonine, and L-cysteine for optimization and maintenance of the intestinal barrier.


How the Triad Works Together


This triple-threat approach supports a healthy gut ecosystem on several fronts:


  • Microbial Diversity & Balance: The combination of probiotics and precision prebiotics helps maintain a rich and harmonious community of beneficial bacteria, supporting both immune and digestive health. Studies have shown this synergy promotes the growth of key species like Akkermansia muciniphila, Faecalibacterium prausnitzii, and Bifidobacteria.
  • Gut Barrier & Immune Support: MegaMucosa™’s unique blend not only supports the integrity of the gut lining, but its immunoglobulins also bolster your mucosal immune defenses. This can help your body better handle occasional digestive discomfort and maintain overall resilience.
  • Short-Chain Fatty Acid Production: Prebiotics and probiotics work together to boost the production of short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate—critical metabolites that nourish the gut lining and help regulate inflammation.
  • Metabolic and Systemic Benefits: Regular use of this kit has been linked in research to support for healthy triglyceride levels, iron balance, and even liver and cardiac function. These benefits stem from a well-tuned gut environment that influences systems throughout the body.
  • Resilience Against Modern Stressors: By reinforcing a strong mucosal barrier and promoting the growth of commensal (friendly) bacteria, this trio helps your gut withstand everyday dietary and environmental challenges.


In summary, this comprehensive gut health toolkit is designed to recondition, reinforce, and restore your gut’s foundation—helping you feel your best from the inside out.


Microbiome Lab's Total Gut Restoration program $365 is the triple threat to help you recover gut health.

TOTAL GUT RESTORATION (3Rs)

Frequently Asked Questions About Gut Health Restoration Program

  • Will the Total Gut Resoration (TGR) get rid of my Celliac?

    TGR does not cure any specific disease, it is designed to balance the microbiome.

  • How long is the Total Gut Restoration (TGR) program?

    The kit consists of three products that are need to complete the initial cycle, however it is advised to continue on the MegaSporeBiotics. Total Gut Restoration is designed as an advanced 3-month protocol to support and restore gut health, giving your body the time it needs to make meaningful changes. While the initial cycle covers these three months, many people choose to maintain ongoing gut support for lasting benefits.

  • Why are there so many different kits for the same program?

    Some people want powder to put with their food and water; while some powders are in capsules.

    In addition, gut health programs often offer a variety of kit formats think capsules, powders, and even gummies so you can choose what fits best with your daily routine and preferences. Capsules are quick and portable for those on the go, while powders can be mixed into smoothies or water for folks who prefer sipping. Lately, gummies have gained popularity, offering a chewable option for adults, kids, or anyone who just doesn't want to swallow pills.

    These different versions can also target specific needs: for example, some kits focus on supporting the gut barrier, others emphasize prebiotics to feed beneficial bacteria, and a few combine several approaches for comprehensive gut restoration. The idea is to make the program accessible and customizable, so you actually stick with it and get your gut back on track without dreading your daily supplement routine.


  • It talks about getting rid of candida; are there other things we should be doing to help the 3Rs Total Gut Restoration (TGR)?

    You can never get rid of candida unless you stop feeding it and it is best to cut their food suppy by stopping simple carbs and sugers.

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